Abstract In this study, we attempted to prepare novel angiotensin I-converting enzyme inhibitory (ACEI) peptides from Tie Guanyin tea residue protein (TTRP). Different proteases were used to hydrolyze TTRP to prepare ACEI peptides, neutral protease and alkaline protease hydrolysates exhibited higher ACEI activity and was chosen in further hydrolysis treatment. Response surface methodology (RSM) was used to optimize the enzymatic condition, and the results indicated that the optimal conditions of enzymatic hydrolysis were: 4% (w/v) of TTRP in water was hydrolyzed with 3500 U/g neutral protease at 50 °C and pH 6.5 for 2.5 h in the first step and then with 5000 U/g alkaline protease at 50 °C and pH 10.0 for 3 h in the second step. Under the optimum condition, the ACE inhibitory rate of the final products was 88.45% ± 0.45% at the concentration of 1.0 mg/mL. Moreover, ACEI peptides derived from TTRP showed good stability under different temperatures, pHs, metal ions and gastrointestinal digestion. These results indicated that the TTRP has potential to be used for preparing ACEI peptides.

Quatro novas dibenzociclooctadienolignanas, neglectalignanas A-D (1-4), juntamente com dezenove compostos conhecidos (5-23), foram isolados das hastes de Schisandra neglecta. Suas estruturas e esteroquímicas foram elucidadas por métodos espectroscópicos, incluindo as técnicas de ressonância magnética nuclear (RMN) de 1D e 2D, e espectrometria de massas de alta resolução com ionização por electrospray (HR-ESI-MS). Avaliou-se as atividades anti-HIV e a citotoxicidade dos compostos 1-4 , e os resultados mostraram que os compostos 1-4 apresentam atividadade anti-HIV moderadas com valores de índice terapêutico (TI) acima de 61,7, 22,6, 57,7 e 27,9, respectivamente, e atividades citotóxicas fracas contra algumas das linhas celulares selecionadas.

Four new dibenzocyclooctadiene lignans, neglectalignans A-D (1-4), together with nineteen known compounds (5-23) were isolated from the stems of Schisandra neglecta. Their structures and stereochemistries were elucidated by spectroscopic methods, including 1D-, 2D-nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) techniques. Compounds 1-4 were evaluated for their anti-HIV activities and cytotoxicities. The results revealed that compounds 1-4 showed moderate anti-HIV-1 activities with therapeutic index (TI) values above 61.7, 22.6, 57.7, and 27.9, respectively, and weak cytotoxic activities for some selected cell lines.